d 10 catalouge Search Results


hvem antibody d 5  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology hvem antibody d 5
<t>HVEM</t> is highly expressed on MM cell lines and primary myeloma cells (A and C) Indicated cancer cell lines were stained with antibodies for (A) NECTIN-1 (CD111) and (C) HVEM (CD270). Overlay images show relative surface NECTIN-1 levels and HVEM levels on different cell lines with respect to their individual isotype control. (B, D, and E) Distribution of (B) NECTIN-1, (D) HVEM, and (E) BCMA expression in 768 newly diagnosed patients with MM from the CoMMpass trial. Density plots of expression are expressed in log 2 (FPKM+1). The first quartile (blue), median (gray), and third quartile (red) of expression are denoted by dashed lines. (F) Representative flow cytometry dot plot demonstrating gating strategy for plasma cells (CD138 + , CD38 + ) and HVEM staining in plasma cells versus non-plasma cells versus unstained cells. (G) Mean florescence intensity of HVEM in plasma cells versus non-plasma cells from 10 myeloma BM aspirates. (H) Cancer types are sorted by median expression for HVEM and include data from the Genomic Data Commons. Boxplots show the median and first and third quartiles of expression, with the whiskers extending to the most extreme data point within 1.5 times the interquartile range. LAML, acute myeloid leukemia; DLBC, lymphoid neoplasm diffuse large B cell lymphoma; ALL, acute lymphoblastic leukemia; BLCA, bladder urothelial carcinoma; BRCA, breast invasive carcinoma; COAD, colon adenocarcinoma; HNSC, head and neck squamous cell carcinoma; KIRC, kidney renal clear cell carcinoma; LGG, brain lower grade glioma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; PRAD, prostate adenocarcinoma; THCA, thyroid carcinoma; UCEC, uterine corpus endometrial carcinoma. See also and and . Data are reported as mean ± SD; n=10, p=0.0002.
Hvem Antibody D 5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/pmc07940704-196-20-26?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
hvem antibody d 5 - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
mab against murine il 18rα  (R&D Systems)
90
R&D Systems mab against murine il 18rα
Figure 1. <t>Anti-IL-18Rα</t> mAb alleviates aGVHD systemic symptoms in aGVHD mice. aGVHD was induced in irradiated BALB/c mice by BMC transplanta- tion. Ten micrograms of anti-IL-18Rα mAb was administered by intraperitoneal injection to mice in the BS+Ab group, while the BS group received injection of PBS. (A) Hunch posture, hair loss and skin lesions were evaluated on day 14 P.T. (B) Changes in body weight of the recipient mice at different time‑points in the different groups. (C) aGVHD clinical scores of the BS+Ab and BS groups on day 14 P.T. (D) Survival rates of aGVHD mice in the different groups. n=6 in each group. *P<0.05. GVHD, graft-versus-host disease; IL-18, interleukin-18; aGVHD, acute GVHD; BMC, bone marrow cell; PBS, phosphate-buffered solution; mAb, monoclonal antibody; P.T., post‑transplantation.
Mab Against Murine Il 18rα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/pm26252430-42-14-21?v=R%26D+Systems
Average 90 stars, based on 1 article reviews
mab against murine il 18rα - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
anti dc sign monoclonal antibodies  (R&D Systems)
93
R&D Systems anti dc sign monoclonal antibodies
Evaluation of <t>DC-SIGN</t> mediated trans infection among H5N1-PVs carrying N-glycosylation mutations. ( A ) The scheme of modified conventional capture assay is demonstrated. ( B ) Raji and Raji-DC-SIGN were used as captured cells. They were incubated with H5N1-PVs at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three to five times) and the target MDCK cells were subjected to luminescence analysis. In addition, for detecting the virions budding from cis infection, the transwell system was used to monitor those virions released from captured cells further causing MDCK (target cells) infection. The lower channel of infected MDCK cells in the transwell were also subjected to luminescence analysis. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN <t>monoclonal</t> antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. ( C ) The Raji and Raji-DC-SIGN cells (captured cells) were incubated with H5N1-PVs carrying different N-glycosylation mutations on HA at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three-five times) and the target MDCK cells were subjected to luminescence analysis. Similarly, the detection of the virions released from cis infection of the captured cells was monitored using transwell system mentioned above. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. The significant difference was measured by each N-glycosylation mutant compared to WT group. Representative results are shown. Quantitative data represent the means ± SD of results from at least three independent experiments (WT, wild-type) (* p < 0.05; ** p < 0.01).
Anti Dc Sign Monoclonal Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/pmc07828482-255-43-48?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
anti dc sign monoclonal antibodies - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
phycoerythrin pe anti osteocalcin  (R&D Systems)
92
R&D Systems phycoerythrin pe anti osteocalcin
Evaluation of <t>DC-SIGN</t> mediated trans infection among H5N1-PVs carrying N-glycosylation mutations. ( A ) The scheme of modified conventional capture assay is demonstrated. ( B ) Raji and Raji-DC-SIGN were used as captured cells. They were incubated with H5N1-PVs at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three to five times) and the target MDCK cells were subjected to luminescence analysis. In addition, for detecting the virions budding from cis infection, the transwell system was used to monitor those virions released from captured cells further causing MDCK (target cells) infection. The lower channel of infected MDCK cells in the transwell were also subjected to luminescence analysis. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN <t>monoclonal</t> antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. ( C ) The Raji and Raji-DC-SIGN cells (captured cells) were incubated with H5N1-PVs carrying different N-glycosylation mutations on HA at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three-five times) and the target MDCK cells were subjected to luminescence analysis. Similarly, the detection of the virions released from cis infection of the captured cells was monitored using transwell system mentioned above. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. The significant difference was measured by each N-glycosylation mutant compared to WT group. Representative results are shown. Quantitative data represent the means ± SD of results from at least three independent experiments (WT, wild-type) (* p < 0.05; ** p < 0.01).
Phycoerythrin Pe Anti Osteocalcin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/pmc09345831-96-15-21?v=R%26D+Systems
Average 92 stars, based on 1 article reviews
phycoerythrin pe anti osteocalcin - by Bioz Stars, 2026-07
92/100 stars
  Buy from Supplier
96 well plates  (Greiner Bio)
99
Greiner Bio 96 well plates
Evaluation of <t>DC-SIGN</t> mediated trans infection among H5N1-PVs carrying N-glycosylation mutations. ( A ) The scheme of modified conventional capture assay is demonstrated. ( B ) Raji and Raji-DC-SIGN were used as captured cells. They were incubated with H5N1-PVs at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three to five times) and the target MDCK cells were subjected to luminescence analysis. In addition, for detecting the virions budding from cis infection, the transwell system was used to monitor those virions released from captured cells further causing MDCK (target cells) infection. The lower channel of infected MDCK cells in the transwell were also subjected to luminescence analysis. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN <t>monoclonal</t> antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. ( C ) The Raji and Raji-DC-SIGN cells (captured cells) were incubated with H5N1-PVs carrying different N-glycosylation mutations on HA at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three-five times) and the target MDCK cells were subjected to luminescence analysis. Similarly, the detection of the virions released from cis infection of the captured cells was monitored using transwell system mentioned above. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. The significant difference was measured by each N-glycosylation mutant compared to WT group. Representative results are shown. Quantitative data represent the means ± SD of results from at least three independent experiments (WT, wild-type) (* p < 0.05; ** p < 0.01).
96 Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/pmc06249570-32-15-20?v=Greiner+Bio
Average 99 stars, based on 1 article reviews
96 well plates - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
biotinylated detection antibody  (R&D Systems)
94
R&D Systems biotinylated detection antibody
Evaluation of <t>DC-SIGN</t> mediated trans infection among H5N1-PVs carrying N-glycosylation mutations. ( A ) The scheme of modified conventional capture assay is demonstrated. ( B ) Raji and Raji-DC-SIGN were used as captured cells. They were incubated with H5N1-PVs at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three to five times) and the target MDCK cells were subjected to luminescence analysis. In addition, for detecting the virions budding from cis infection, the transwell system was used to monitor those virions released from captured cells further causing MDCK (target cells) infection. The lower channel of infected MDCK cells in the transwell were also subjected to luminescence analysis. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN <t>monoclonal</t> antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. ( C ) The Raji and Raji-DC-SIGN cells (captured cells) were incubated with H5N1-PVs carrying different N-glycosylation mutations on HA at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three-five times) and the target MDCK cells were subjected to luminescence analysis. Similarly, the detection of the virions released from cis infection of the captured cells was monitored using transwell system mentioned above. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. The significant difference was measured by each N-glycosylation mutant compared to WT group. Representative results are shown. Quantitative data represent the means ± SD of results from at least three independent experiments (WT, wild-type) (* p < 0.05; ** p < 0.01).
Biotinylated Detection Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/pmc05214528-95-25-37?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
biotinylated detection antibody - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
protein 10  (R&D Systems)
96
R&D Systems protein 10
Evaluation of <t>DC-SIGN</t> mediated trans infection among H5N1-PVs carrying N-glycosylation mutations. ( A ) The scheme of modified conventional capture assay is demonstrated. ( B ) Raji and Raji-DC-SIGN were used as captured cells. They were incubated with H5N1-PVs at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three to five times) and the target MDCK cells were subjected to luminescence analysis. In addition, for detecting the virions budding from cis infection, the transwell system was used to monitor those virions released from captured cells further causing MDCK (target cells) infection. The lower channel of infected MDCK cells in the transwell were also subjected to luminescence analysis. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN <t>monoclonal</t> antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. ( C ) The Raji and Raji-DC-SIGN cells (captured cells) were incubated with H5N1-PVs carrying different N-glycosylation mutations on HA at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three-five times) and the target MDCK cells were subjected to luminescence analysis. Similarly, the detection of the virions released from cis infection of the captured cells was monitored using transwell system mentioned above. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. The significant difference was measured by each N-glycosylation mutant compared to WT group. Representative results are shown. Quantitative data represent the means ± SD of results from at least three independent experiments (WT, wild-type) (* p < 0.05; ** p < 0.01).
Protein 10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/pm39508142-46-20-27?v=R%26D+Systems
Average 96 stars, based on 1 article reviews
protein 10 - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
cxcl10 ip 10  (R&D Systems)
96
R&D Systems cxcl10 ip 10
Evaluation of <t>DC-SIGN</t> mediated trans infection among H5N1-PVs carrying N-glycosylation mutations. ( A ) The scheme of modified conventional capture assay is demonstrated. ( B ) Raji and Raji-DC-SIGN were used as captured cells. They were incubated with H5N1-PVs at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three to five times) and the target MDCK cells were subjected to luminescence analysis. In addition, for detecting the virions budding from cis infection, the transwell system was used to monitor those virions released from captured cells further causing MDCK (target cells) infection. The lower channel of infected MDCK cells in the transwell were also subjected to luminescence analysis. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN <t>monoclonal</t> antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. ( C ) The Raji and Raji-DC-SIGN cells (captured cells) were incubated with H5N1-PVs carrying different N-glycosylation mutations on HA at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three-five times) and the target MDCK cells were subjected to luminescence analysis. Similarly, the detection of the virions released from cis infection of the captured cells was monitored using transwell system mentioned above. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. The significant difference was measured by each N-glycosylation mutant compared to WT group. Representative results are shown. Quantitative data represent the means ± SD of results from at least three independent experiments (WT, wild-type) (* p < 0.05; ** p < 0.01).
Cxcl10 Ip 10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/bio_rxiv__64898__2026__04__06__716685-190-8-9?v=R%26D+Systems
Average 96 stars, based on 1 article reviews
cxcl10 ip 10 - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
il 6  (R&D Systems)
96
R&D Systems il 6
Evaluation of <t>DC-SIGN</t> mediated trans infection among H5N1-PVs carrying N-glycosylation mutations. ( A ) The scheme of modified conventional capture assay is demonstrated. ( B ) Raji and Raji-DC-SIGN were used as captured cells. They were incubated with H5N1-PVs at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three to five times) and the target MDCK cells were subjected to luminescence analysis. In addition, for detecting the virions budding from cis infection, the transwell system was used to monitor those virions released from captured cells further causing MDCK (target cells) infection. The lower channel of infected MDCK cells in the transwell were also subjected to luminescence analysis. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN <t>monoclonal</t> antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. ( C ) The Raji and Raji-DC-SIGN cells (captured cells) were incubated with H5N1-PVs carrying different N-glycosylation mutations on HA at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three-five times) and the target MDCK cells were subjected to luminescence analysis. Similarly, the detection of the virions released from cis infection of the captured cells was monitored using transwell system mentioned above. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. The significant difference was measured by each N-glycosylation mutant compared to WT group. Representative results are shown. Quantitative data represent the means ± SD of results from at least three independent experiments (WT, wild-type) (* p < 0.05; ** p < 0.01).
Il 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/pmc09287136-98-8-11?v=R%26D+Systems
Average 96 stars, based on 1 article reviews
il 6 - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
stock il 2  (R&D Systems)
96
R&D Systems stock il 2
Cellular activity of Jak3 inhibitors. Upper panel, phosphorylation of STAT5 (pSTAT5) <t>in</t> <t>IL-2-stimulated</t> T-blasts (dependent on signaling from both Jak1 and Jak3). Lower panel, phosphorylation of STAT5 in EPO-stimulated UT-7 cells (dependent on signaling from Jak2). Covalent tricyclic compounds (Compounds (Cmpd) 1 and 3) are inactive (>50 μm) in this assay. Error bars, S.E.
Stock Il 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/pmc04335200-555-12-14?v=R%26D+Systems
Average 96 stars, based on 1 article reviews
stock il 2 - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
higf 1r hetero tetramere  (R&D Systems)
92
R&D Systems higf 1r hetero tetramere
Cellular activity of Jak3 inhibitors. Upper panel, phosphorylation of STAT5 (pSTAT5) <t>in</t> <t>IL-2-stimulated</t> T-blasts (dependent on signaling from both Jak1 and Jak3). Lower panel, phosphorylation of STAT5 in EPO-stimulated UT-7 cells (dependent on signaling from Jak2). Covalent tricyclic compounds (Compounds (Cmpd) 1 and 3) are inactive (>50 μm) in this assay. Error bars, S.E.
Higf 1r Hetero Tetramere, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/us11365259-54-16-35?v=R%26D+Systems
Average 92 stars, based on 1 article reviews
higf 1r hetero tetramere - by Bioz Stars, 2026-07
92/100 stars
  Buy from Supplier
banana-flavored pellets  (Bio-Serv)
90
Bio-Serv banana-flavored pellets
Cellular activity of Jak3 inhibitors. Upper panel, phosphorylation of STAT5 (pSTAT5) <t>in</t> <t>IL-2-stimulated</t> T-blasts (dependent on signaling from both Jak1 and Jak3). Lower panel, phosphorylation of STAT5 in EPO-stimulated UT-7 cells (dependent on signaling from Jak2). Covalent tricyclic compounds (Compounds (Cmpd) 1 and 3) are inactive (>50 μm) in this assay. Error bars, S.E.
Banana Flavored Pellets, supplied by Bio-Serv, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+10+catalouge/pmc03756749-168-10-15?v=Bio-Serv
Average 90 stars, based on 1 article reviews
banana-flavored pellets - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


HVEM is highly expressed on MM cell lines and primary myeloma cells (A and C) Indicated cancer cell lines were stained with antibodies for (A) NECTIN-1 (CD111) and (C) HVEM (CD270). Overlay images show relative surface NECTIN-1 levels and HVEM levels on different cell lines with respect to their individual isotype control. (B, D, and E) Distribution of (B) NECTIN-1, (D) HVEM, and (E) BCMA expression in 768 newly diagnosed patients with MM from the CoMMpass trial. Density plots of expression are expressed in log 2 (FPKM+1). The first quartile (blue), median (gray), and third quartile (red) of expression are denoted by dashed lines. (F) Representative flow cytometry dot plot demonstrating gating strategy for plasma cells (CD138 + , CD38 + ) and HVEM staining in plasma cells versus non-plasma cells versus unstained cells. (G) Mean florescence intensity of HVEM in plasma cells versus non-plasma cells from 10 myeloma BM aspirates. (H) Cancer types are sorted by median expression for HVEM and include data from the Genomic Data Commons. Boxplots show the median and first and third quartiles of expression, with the whiskers extending to the most extreme data point within 1.5 times the interquartile range. LAML, acute myeloid leukemia; DLBC, lymphoid neoplasm diffuse large B cell lymphoma; ALL, acute lymphoblastic leukemia; BLCA, bladder urothelial carcinoma; BRCA, breast invasive carcinoma; COAD, colon adenocarcinoma; HNSC, head and neck squamous cell carcinoma; KIRC, kidney renal clear cell carcinoma; LGG, brain lower grade glioma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; PRAD, prostate adenocarcinoma; THCA, thyroid carcinoma; UCEC, uterine corpus endometrial carcinoma. See also and and . Data are reported as mean ± SD; n=10, p=0.0002.

Journal: Molecular Therapy Oncolytics

Article Title: Oncolytic herpes simplex virus infects myeloma cells in vitro and in vivo

doi: 10.1016/j.omto.2021.02.009

Figure Lengend Snippet: HVEM is highly expressed on MM cell lines and primary myeloma cells (A and C) Indicated cancer cell lines were stained with antibodies for (A) NECTIN-1 (CD111) and (C) HVEM (CD270). Overlay images show relative surface NECTIN-1 levels and HVEM levels on different cell lines with respect to their individual isotype control. (B, D, and E) Distribution of (B) NECTIN-1, (D) HVEM, and (E) BCMA expression in 768 newly diagnosed patients with MM from the CoMMpass trial. Density plots of expression are expressed in log 2 (FPKM+1). The first quartile (blue), median (gray), and third quartile (red) of expression are denoted by dashed lines. (F) Representative flow cytometry dot plot demonstrating gating strategy for plasma cells (CD138 + , CD38 + ) and HVEM staining in plasma cells versus non-plasma cells versus unstained cells. (G) Mean florescence intensity of HVEM in plasma cells versus non-plasma cells from 10 myeloma BM aspirates. (H) Cancer types are sorted by median expression for HVEM and include data from the Genomic Data Commons. Boxplots show the median and first and third quartiles of expression, with the whiskers extending to the most extreme data point within 1.5 times the interquartile range. LAML, acute myeloid leukemia; DLBC, lymphoid neoplasm diffuse large B cell lymphoma; ALL, acute lymphoblastic leukemia; BLCA, bladder urothelial carcinoma; BRCA, breast invasive carcinoma; COAD, colon adenocarcinoma; HNSC, head and neck squamous cell carcinoma; KIRC, kidney renal clear cell carcinoma; LGG, brain lower grade glioma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; PRAD, prostate adenocarcinoma; THCA, thyroid carcinoma; UCEC, uterine corpus endometrial carcinoma. See also and and . Data are reported as mean ± SD; n=10, p=0.0002.

Article Snippet: 1 × 10 6 MM cells (MM.1S, NCI-H929, RPMI 8266, and L363) were incubated in growth medium with 10 μg/mL HVEM antibody (D-5) (catalog no. sc-365971, Santa Cruz) and/or NECTIN-1 antibody (F-10) (catalog no. sc-271063, Santa Cruz) for 30 min, then infected with an MOI of 0.1 oHSV-1.

Techniques: Staining, Control, Expressing, Flow Cytometry, Clinical Proteomics

Figure 1. Anti-IL-18Rα mAb alleviates aGVHD systemic symptoms in aGVHD mice. aGVHD was induced in irradiated BALB/c mice by BMC transplanta- tion. Ten micrograms of anti-IL-18Rα mAb was administered by intraperitoneal injection to mice in the BS+Ab group, while the BS group received injection of PBS. (A) Hunch posture, hair loss and skin lesions were evaluated on day 14 P.T. (B) Changes in body weight of the recipient mice at different time‑points in the different groups. (C) aGVHD clinical scores of the BS+Ab and BS groups on day 14 P.T. (D) Survival rates of aGVHD mice in the different groups. n=6 in each group. *P<0.05. GVHD, graft-versus-host disease; IL-18, interleukin-18; aGVHD, acute GVHD; BMC, bone marrow cell; PBS, phosphate-buffered solution; mAb, monoclonal antibody; P.T., post‑transplantation.

Journal: Oncology reports

Article Title: Protective effect of neutralizing anti-IL-18α monoclonal antibody on a mouse model of acute graft-versus-host disease.

doi: 10.3892/or.2015.4176

Figure Lengend Snippet: Figure 1. Anti-IL-18Rα mAb alleviates aGVHD systemic symptoms in aGVHD mice. aGVHD was induced in irradiated BALB/c mice by BMC transplanta- tion. Ten micrograms of anti-IL-18Rα mAb was administered by intraperitoneal injection to mice in the BS+Ab group, while the BS group received injection of PBS. (A) Hunch posture, hair loss and skin lesions were evaluated on day 14 P.T. (B) Changes in body weight of the recipient mice at different time‑points in the different groups. (C) aGVHD clinical scores of the BS+Ab and BS groups on day 14 P.T. (D) Survival rates of aGVHD mice in the different groups. n=6 in each group. *P<0.05. GVHD, graft-versus-host disease; IL-18, interleukin-18; aGVHD, acute GVHD; BMC, bone marrow cell; PBS, phosphate-buffered solution; mAb, monoclonal antibody; P.T., post‑transplantation.

Article Snippet: Recipient mice in the BS+Ab group also received 10 μg/mouse intraperitoneal injection of neutralizing mAb against murine IL-18Rα (catalog no. MAB12161; R&D Systems, Minneapolis, MN, uSA) every 2 days.

Techniques: Irradiation, Injection

Figure 2. Effect of anti-IL-18Rα mAb administration on Th cell subsets, pro-inflammatory cytokines and histological scores in the aGVHD mice. (A-C) Peripheral blood levels of Th1 (A), Th2 (B) and Th17 (C) cell subsets in the BS+Ab and BS experimental groups were measured by flow cytometry at different time-points. Serum levels of IFN-γ (D), IL-4 (E), IL-17A (F) and IL-6 (H) at different time-points were detected by cytometric bead array, and IL-18 levels (G) were measured by ELISA. (I) Representative H&E staining of the liver and small intestine tissues of the mice in the BS+Ab and BS groups and the normal control group (untreated animals) on day 14 P.T. Magnification, x400. (J) Histological score was measured on day 14 P.T. n=6 in each group, *P<0.05. GVHD, graft-versus-host disease; aGVHD, acute GVHD; mAb, monoclonal antibody; Th, T helper; IL-18, interleukin-18; ELISA, enzyme-linked immunosorbent assay; H&E, hematoxylin and eosin; P.T., post‑transplantation.

Journal: Oncology reports

Article Title: Protective effect of neutralizing anti-IL-18α monoclonal antibody on a mouse model of acute graft-versus-host disease.

doi: 10.3892/or.2015.4176

Figure Lengend Snippet: Figure 2. Effect of anti-IL-18Rα mAb administration on Th cell subsets, pro-inflammatory cytokines and histological scores in the aGVHD mice. (A-C) Peripheral blood levels of Th1 (A), Th2 (B) and Th17 (C) cell subsets in the BS+Ab and BS experimental groups were measured by flow cytometry at different time-points. Serum levels of IFN-γ (D), IL-4 (E), IL-17A (F) and IL-6 (H) at different time-points were detected by cytometric bead array, and IL-18 levels (G) were measured by ELISA. (I) Representative H&E staining of the liver and small intestine tissues of the mice in the BS+Ab and BS groups and the normal control group (untreated animals) on day 14 P.T. Magnification, x400. (J) Histological score was measured on day 14 P.T. n=6 in each group, *P<0.05. GVHD, graft-versus-host disease; aGVHD, acute GVHD; mAb, monoclonal antibody; Th, T helper; IL-18, interleukin-18; ELISA, enzyme-linked immunosorbent assay; H&E, hematoxylin and eosin; P.T., post‑transplantation.

Article Snippet: Recipient mice in the BS+Ab group also received 10 μg/mouse intraperitoneal injection of neutralizing mAb against murine IL-18Rα (catalog no. MAB12161; R&D Systems, Minneapolis, MN, uSA) every 2 days.

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay, Staining, Control

Evaluation of DC-SIGN mediated trans infection among H5N1-PVs carrying N-glycosylation mutations. ( A ) The scheme of modified conventional capture assay is demonstrated. ( B ) Raji and Raji-DC-SIGN were used as captured cells. They were incubated with H5N1-PVs at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three to five times) and the target MDCK cells were subjected to luminescence analysis. In addition, for detecting the virions budding from cis infection, the transwell system was used to monitor those virions released from captured cells further causing MDCK (target cells) infection. The lower channel of infected MDCK cells in the transwell were also subjected to luminescence analysis. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. ( C ) The Raji and Raji-DC-SIGN cells (captured cells) were incubated with H5N1-PVs carrying different N-glycosylation mutations on HA at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three-five times) and the target MDCK cells were subjected to luminescence analysis. Similarly, the detection of the virions released from cis infection of the captured cells was monitored using transwell system mentioned above. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. The significant difference was measured by each N-glycosylation mutant compared to WT group. Representative results are shown. Quantitative data represent the means ± SD of results from at least three independent experiments (WT, wild-type) (* p < 0.05; ** p < 0.01).

Journal: International Journal of Molecular Sciences

Article Title: Identification of Important N-Linked Glycosylation Sites in the Hemagglutinin Protein and Their Functional Impact on DC-SIGN Mediated Avian Influenza H5N1 Infection

doi: 10.3390/ijms22020743

Figure Lengend Snippet: Evaluation of DC-SIGN mediated trans infection among H5N1-PVs carrying N-glycosylation mutations. ( A ) The scheme of modified conventional capture assay is demonstrated. ( B ) Raji and Raji-DC-SIGN were used as captured cells. They were incubated with H5N1-PVs at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three to five times) and the target MDCK cells were subjected to luminescence analysis. In addition, for detecting the virions budding from cis infection, the transwell system was used to monitor those virions released from captured cells further causing MDCK (target cells) infection. The lower channel of infected MDCK cells in the transwell were also subjected to luminescence analysis. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. ( C ) The Raji and Raji-DC-SIGN cells (captured cells) were incubated with H5N1-PVs carrying different N-glycosylation mutations on HA at 4 °C for 2 h and then co-cultured with MDCK (target cells) at 37 °C for 24–48 h. After co-culturing, the capture cells were removed via intensive PBS washing (three-five times) and the target MDCK cells were subjected to luminescence analysis. Similarly, the detection of the virions released from cis infection of the captured cells was monitored using transwell system mentioned above. Alternatively, some groups were co-treated with IgG control and anti-DC-SIGN monoclonal antibodies. The relative infectivity was measured by using the luminescence values of co-cultured MDCK, normalized with values of MDCK from transwell system. The significant difference was measured by each N-glycosylation mutant compared to WT group. Representative results are shown. Quantitative data represent the means ± SD of results from at least three independent experiments (WT, wild-type) (* p < 0.05; ** p < 0.01).

Article Snippet: For a DC-SIGN-enhanced infectivity assay, a 5 × 10 5 susceptible cells mentioned above were seeded into 48-well plates prior to incubation with H5N1 pseudotyped or H5N1-RG virus particles at 37 °C for 2 h. Alternatively, some of these cells were pretreated with anti-DC-SIGN monoclonal antibodies (10 μg/mL-1; R&D System, catalog no. MAB161).

Techniques: Infection, Glycoproteomics, Modification, Incubation, Cell Culture, Control, Bioprocessing, Mutagenesis

Cellular activity of Jak3 inhibitors. Upper panel, phosphorylation of STAT5 (pSTAT5) in IL-2-stimulated T-blasts (dependent on signaling from both Jak1 and Jak3). Lower panel, phosphorylation of STAT5 in EPO-stimulated UT-7 cells (dependent on signaling from Jak2). Covalent tricyclic compounds (Compounds (Cmpd) 1 and 3) are inactive (>50 μm) in this assay. Error bars, S.E.

Journal: The Journal of Biological Chemistry

Article Title: Tricyclic Covalent Inhibitors Selectively Target Jak3 through an Active Site Thiol *

doi: 10.1074/jbc.M114.595181

Figure Lengend Snippet: Cellular activity of Jak3 inhibitors. Upper panel, phosphorylation of STAT5 (pSTAT5) in IL-2-stimulated T-blasts (dependent on signaling from both Jak1 and Jak3). Lower panel, phosphorylation of STAT5 in EPO-stimulated UT-7 cells (dependent on signaling from Jak2). Covalent tricyclic compounds (Compounds (Cmpd) 1 and 3) are inactive (>50 μm) in this assay. Error bars, S.E.

Article Snippet: Cells were then stimulated by adding 2.5 μl/well of 80 ng/ml (4× stock) IL-2 (R&D Systems catalog no. 202-IL-10) and incubated for 20 min. pSTAT5 was assessed as described above for the EPO assay.

Techniques: Activity Assay